Thesis Details
Predikce vlivu mutace na rozpustnost proteinů
Protein solubility is a key problem in production of functional proteins. Prediction of the effect of mutation on protein solubility could save a lot of time and money, as it would provide in silico prediction of solubility enhancing mutations before performing deep mutational scanning in laboratory. In this work, new predictor of the effect of mutation on protein solubility SoluProtMut is introduced that is based on machine learning methods. Most of the existing predictors predict the effect from the amino acid sequence. In addition to the sequence, the tool presented in this work also uses the spatial structure of the protein, which can significantly increase it's accuracy.
solubility, protein, mutation, prediction, machine learning, SoluProtMut
Beran Vítězslav, doc. Ing., Ph.D. (DCGM FIT BUT), člen
Holík Lukáš, doc. Mgr., Ph.D. (DITS FIT BUT), člen
Jaroš Jiří, doc. Ing., Ph.D. (DCSY FIT BUT), člen
Křivka Zbyněk, Ing., Ph.D. (DIFS FIT BUT), člen
@bachelorsthesis{FITBT22864,
author = "J\'{u}lius Marko",
type = "Bachelor's thesis",
title = "Predikce vlivu mutace na rozpustnost protein\r{u}",
school = "Brno University of Technology, Faculty of Information Technology",
year = 2020,
location = "Brno, CZ",
language = "slovak",
url = "https://www.fit.vut.cz/study/thesis/22864/"
}