Publication Details

FireProt: web server for automated design of thermostable proteins

MUSIL Miloš, ŠTOURAČ Jan, BENDL Jaroslav, BREZOVSKÝ Jan, PROKOP Zbyněk, ZENDULKA Jaroslav, MARTÍNEK Tomáš, BEDNÁŘ David and DAMBORSKÝ Jiří. FireProt: web server for automated design of thermostable proteins. Nucleic Acids Research, vol. 2017, no. 45, pp. 393-399. ISSN 1362-4962.
Czech title
FireProt: web server pro automatizovaný návrh termostabilních proteinů
journal article
Musil Miloš, Ing. (DIFS FIT BUT)
Štourač Jan (LL)
Bendl Jaroslav, Ing. (DIFS FIT BUT)
Brezovský Jan, Mgr., Ph.D. (LL)
Prokop Zbyněk, doc. RnDr., Ph.D. (LL)
Zendulka Jaroslav, doc. Ing., CSc. (DIFS FIT BUT)
Martínek Tomáš, Ing., Ph.D. (DCSY FIT BUT)
Bednář David, Mgr. (LL)
Damborský Jiří, prof. Mgr., Dr. (LL)
mutations, protein engineering, mutant proteins, protein stability, thermostable proteins
There is a continuous interest in increasing proteins stability to enhance their usability in numerous biomedical and biotechnological applications. A number of in silico tools for the prediction of the effect of mutations on protein stability have been developed recently. However, only single-point mutations with a small effect on protein stability are typically predicted with the existing tools and have to be followed by laborious protein expression, purification, and characterization. Here, we present FireProt, a web server for the automated design of multiplepoint thermostable mutant proteins that combines structural and evolutionary information in its calculation core. FireProt utilizes sixteen tools and three protein engineering strategies for making reliable protein designs. The server is complemented with interactive, easy-to-use interface that allows users to directly analyze and optionally modify designed thermostable mutants. FireProt is freely available at
Nucleic Acids Research, vol. 2017, no. 45, ISSN 1362-4962
Oxford University Press
   author = "Milo\v{s} Musil and Jan \v{S}toura\v{c} and Jaroslav Bendl and Jan Brezovsk\'{y} and Zbyn\v{e}k Prokop and Jaroslav Zendulka and Tom\'{a}\v{s} Mart\'{i}nek and David Bedn\'{a}\v{r} and Ji\v{r}\'{i} Damborsk\'{y}",
   title = "FireProt: web server for automated design of thermostable proteins",
   pages = "393--399",
   journal = "Nucleic Acids Research",
   volume = 2017,
   number = 45,
   year = 2017,
   ISSN = "1362-4962",
   doi = "10.1093/nar/gkx285",
   language = "english",
   url = ""
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