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Satellite DNA and Transposable Elements in Seabuckthorn ( Hippophae rhamnoides ), a Dioecious Plant with Small Y and Large X Chromosomes

PUTEROVÁ Janka, RAZUMOVA Olga, MARTÍNEK Tomáš, ALEXANDROV Oleg, DIVASHUK Mikhail, KUBÁT Zdeněk, HOBZA Roman, KARLOV Gennady and KEJNOVSKÝ Eduard. Satellite DNA and Transposable Elements in Seabuckthorn ( Hippophae rhamnoides ), a Dioecious Plant with Small Y and Large X Chromosomes. Genome Biology and Evolution, vol. 9, no. 1, pp. 197-212. ISSN 1759-6653. Available from: https://academic.oup.com/gbe/article/9/1/197/2830930/Satellite-DNA-and-Transposable-Elements-in
Czech title
Satelitní DNA a transpozibilní elementy u rakytníku (Hippophae rhamnoides), dvoudomá rostlina s malým Y a velkými X chromosomy
Type
journal article
Language
english
Authors
Puterová Janka, Ing. (DIFS FIT BUT)
Razumova Olga (MTAA)
Martínek Tomáš, Ing., Ph.D. (DCSY FIT BUT)
Alexandrov Oleg (MTAA)
Divashuk Mikhail (MTAA)
Kubát Zdeněk, Mgr., Ph.D. (IBP)
Hobza Roman (IBP)
Karlov Gennady (MTAA)
Kejnovský Eduard, doc. RNDr., CSc. (SCI MUNI)
URL
Keywords
sex chromosomes, genome composition, chromosomal localization, repetitive DNA
Abstract

Seabuckthorn (Hippophae rhamnoides) is a dioecious shrub commonly used in the pharmaceutical, cosmetic, and environmental
industry as a source of oil, minerals and vitamins. In this study, we analyzed the transposable elements and satellites in its genome.We
carried out Illumina DNA sequencing and reconstructed the main repetitive DNA sequences. For data analysis, we developed a new
bioinformatics approach for advanced satellite DNA analysis and showed that about 25% of the genome consists of satellite DNA and
about 24% is formed of transposable elements, dominated by Ty3/Gypsy and Ty1/Copia LTR retrotransposons. FISH mapping
revealed X chromosome-accumulated, Y chromosome-specific or both sex chromosomes-accumulated satellites but most satellites
were found on autosomes. Transposable elements were located mostly in the subtelomeres of all chromosomes. The 5S rDNA and
45S rDNA were localized on one autosomal locus each. Although we demonstrated the small size of the Y chromosome of the
seabuckthorn and accumulated satellite DNA there, we were unable to estimate the age and extent of the Y chromosome degeneration.
Analysis of dioecious relatives such as Shepherdia would shed more light on the evolution of these sex chromosomes.
Published
2017
Pages
197-212
Journal
Genome Biology and Evolution, vol. 9, no. 1, ISSN 1759-6653
Publisher
Oxford University Press
DOI
BibTeX
@ARTICLE{FITPUB11412,
   author = "Janka Puterov\'{a} and Olga Razumova and Tom\'{a}\v{s} Mart\'{i}nek and Oleg Alexandrov and Mikhail Divashuk and Zden\v{e}k Kub\'{a}t and Roman Hobza and Gennady Karlov and Eduard Kejnovsk\'{y}",
   title = "Satellite DNA and Transposable Elements in Seabuckthorn ( Hippophae rhamnoides ), a Dioecious Plant with Small Y and Large X Chromosomes",
   pages = "197--212",
   journal = "Genome Biology and Evolution",
   volume = 9,
   number = 1,
   year = 2017,
   ISSN = "1759-6653",
   doi = "10.1093/gbe/evw303",
   language = "english",
   url = "https://www.fit.vut.cz/research/publication/11412"
}
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