Thesis Details
Fully Automated Method for the Design of Ancestral Proteins
Protein stability is a crucial feature for the industrial applications of proteins. This thesis focuses on the enhancements of FireProtASR, an automated tool for the design of stable proteins via ancestral sequence reconstruction. A novel technique of the successor prediction was developed and implemented into FireProtASR. Successors represent the evolutionary future of protein sequences and are supposed to have higher activity than current day proteins. They are also expected to have higher ability to target specific molecules. Furthermore, a new web user interface of FireProtASR was developed. It provides a fast and intuitive way to control the tool.
protein engineering, protein stability, stability prediction, ancestral sequence reconstruction, successors
Bidlo Michal, doc. Ing., Ph.D. (DCSY FIT BUT), člen
Burgetová Ivana, Ing., Ph.D. (DIFS FIT BUT), člen
Lengál Ondřej, Ing., Ph.D. (DITS FIT BUT), člen
Matoušek Radomil, doc. Ing., Ph.D. (IACS FME BUT), člen
Vašíček Zdeněk, doc. Ing., Ph.D. (DCSY FIT BUT), člen
@mastersthesis{FITMT24649, author = "Martin \v{S}t\v{e}p\'{a}nek", type = "Master's thesis", title = "Fully Automated Method for the Design of Ancestral Proteins", school = "Brno University of Technology, Faculty of Information Technology", year = 2022, location = "Brno, CZ", language = "english", url = "https://www.fit.vut.cz/study/thesis/24649/" }