Automated Electrochemical Detection of Iron Ions in Erythrocytes from MeLiM Minipigs Suffering from Melanoma

Modern analytical methods allow the detection of compounds of interest in biological samples in very low concentrations. However, some of these compounds are electro-active or provide electro-active complexes after derivatization. In this case, electrochemical detection is very useful. Stationary electrochemical analysers have a plenty of advantages, such as low costs, high sensitivity and repeatability. On the other hand, non-automated injection of samples belongs to the main disadvantages of electrochemical techniques. Due to this fact, we focused on testing of new electrochemical system enabling us to inject units of l of a sample and cooled sample-rack area. The analyser can be used for detection and quantification of a wide range of compounds including metals, peptides, proteins, nucleic acids and drugs. To test the possibilities of suggested system, concentration of iron in blood samples of MeLiM minipigs was measured. Primarily, we optimized the following experimental conditions as supporting electrolyte 0.1 M KBrO3 + 0.3 M NaOH + 0.01 M TEA of pH 11, and deposition potential of 0 V. Moreover, preparation of erythrocytes was optimized. Changes in iron content of control group (10 ug Fe ions/ml) and group of animals suffering from melanoma progression (3.5 ug Fe ions/ml) were determined.